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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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neo_2019_190429N378

Title: PAX3 silencing suppresses gastric cancer proliferation and angiogenesis via MET/PI3K signaling
Author: W.L. Lv, Y.Y. Hu, Z.N. Li, W. Zhang, Q. Pan

Abstract: PAX3 is the key factors in cell signal transduction pathway and may be involved in the regulation of cancer cell proliferation, differentiation, and migration. The aim of the study is to investigate the effects and mechanism of PAX3 silencing on the gastric cancer. Specific PAX3 silencing was performed both in vitro and in vivo using small-interfering RNAs (siRNAs). The proliferation, apoptosis and angiogenesis of gastric cancer cells were assessed using MTT assay, flow cytometry and in vitro tube formation assay. Mice with gastric xenografts, which expressed either si-PAX3 or non-coding siRNA (si-NC), were constructed and the effects of PAX3 silencing on tumor progression were evaluated. PCNA is a proliferating cell nuclear antigen and can be used as an index for evaluating cell proliferation status. Immunocytochemistry assay was used to quantify PAX3 and PCNA expression. After 4 weeks of tumor inoculation, tumor tissues were weighed. Tumor tissue morphology and apoptosis were evaluated using HE staining and TUNEL assay. In order to investigate the effect of silencing PAX3 on cell apoptosis, angiogenesis and MET/PI3K pathway, quantitative real-time PCR (qRT-PCR) or western blot were used to detect the expression levels of caspase-3, VEGF, MET, p-MET, PI3K and p-PI3K. After PAX3 silencing, PAX3 expression was significantly decreased in two gastric cancer cell lines, MKN-28 and SGC-7901 (p

Keywords: PAX3; angiogenesis; gastric cancer
Published online: 17-Dec-2019
Year: , Volume: , Issue: Page From: , Page To:
doi:10.4149/neo_2019_190429N378


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