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ISSN 0028-2685
ISSN 1338-4317 (online)

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neo_2020_190509N412

Title: Long non-coding RNA SOX21-AS1 promotes cell proliferation and invasion through upregulating PAK7 expression by sponging miR-144-3p in glioma cells
Author: S. Y. Gai, Z. H. Yuan

Abstract: In this study, the function of long non-coding RNA SOX21 antisense RNA 1 (SOX21-AS1) in progress of glioma was explored. RNA and protein levels were measured via quantitative reverse transcription-PCR (qRT-PCR) and western blot analysis. In addition, we examined cell proliferation, apoptosis, migration and invasion. The interaction between SOX21-AS1 (PAK7) and miR-144-3p was determined via RNA immunoprecipitation (RIP) assay and Luciferase reporter assay. SOX21-AS1 was upregulated in glioma tissues and cells. SOX21-AS1 knockdown was carried out in glioma cells (U251 and U87 cells). Moreover, in vitro, SOX21-AS1 knockdown repressed proliferation, migration, invasion, and enhanced apoptosis in glioma cells. In vivo, SOX21-AS1 knockdown suppressed tumor growth in mice. In addition, SOX21-AS1 could sponge miR-144-3p, which was determined to bind to PAK7. miR-144-3p knockdown promoted proliferation, migration, invasion, and inhibited cell apoptosis. Importantly, the effects of SOX21-AS1 knockdown-induced proliferation, migration, invasion, and apoptosis were alleviated in glioma cells co-transfected with SOX21-AS1 and miR-144-3p knockdown. Furthermore, miR-144-3p knockdown also attenuated Wnt/β-catenin pathway-associated protein levels induced by SOX21-AS1 knockdown.
These results indicated that SOX21-AS1/miR-144-3p/PAK7 axis played an oncogenic role in glioma cells by regulating Wnt/β-catenin pathway, which suggests a rational therapeutic strategy for glioma.

Keywords: glioma, SOX21-AS1, miR-144-3p, PAK7, migration, invasion
Published online: 22-Jan-2020
Year: , Volume: , Issue: Page From: , Page To:
doi:10.4149/neo_2020_190509N412


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