Home Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.2, p.111–116,2020

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.2

 

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Bratislava Medical Journal Vol.121, No.2, p.111–116,2020

Title: Inhibition of voltage‑gated potassium channels affect expressions of miR-126 and miR-126* in breast cancer cell lines
Author: D. Turgut Cosan, A. Soyocak, C. Oner

Abstract: AIM: We aimed to determine the possible correlation between voltage‑gated potassium channels and micro RNAs in breast cancer and metastatic breast cancer cells.
METHOD: Kv1.3 and Kv10.1 channels were inhibited by specific siRNAs using a lipofectamine-based transfection in MCF-7 and MDA-MB-231 cells. After transfection, total RNA was isolated, and then miR-126 and miR-126* expressions were observed using RT-PCR.
RESULTS: There was a negative correlation between Kv channels and miRNAs according to the characteristics of the breast cancer cells. The inhibition was observed not only in Kv1.3 but also in Kv10.1 in MCF-7 cells, and miR-126 and miR-126* expressions were downregulated compared to the control group (p < 0.001). The inhibition of these channels in MDA-MB-231 cells caused an upregulation of miR-126 and miR-126* expressions (p < 0.001).
CONCLUSION: The miR-126 and miR-126* expressions differed according to benign and malign breast cancer cell lines. Furthermore, we found that miR-126/126* may interact with Kv1.3 and Kv10.1 voltage-gated potassium channels. Our study suggests and indicates the relationship between Kv channels and miRNAs in breast cancer cells (Tab. 1, Fig. 2, Ref. 51).

Keywords: breast cancer, Kv1.3, Kv10.1, miR-126, miR-126*
Published online: 22-Jan-2020
Year: 2020, Volume: 121, Issue: 2 Page From: 111, Page To: 116
doi:10.4149/BLL_2020_014


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