Home 
HOME Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.2, p.111–116,2020
HOME Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.2, p.111–116,2020
Journal info
|
||||
Select Journal
Journals
Bratislava Medical Journal 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 Endocrine Regulations General Physiology and Biophysics Neoplasma Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Bratislava Medical Journal Vol.121, No.2, p.111–116,2020 |
||
| Title: Inhibition of voltage‑gated potassium channels affect expressions of miR-126 and miR-126* in breast cancer cell lines | ||
| Author: D. Turgut Cosan, A. Soyocak, C. Oner | ||
| Abstract: AIM: We aimed to determine the possible correlation between voltage‑gated potassium channels and micro RNAs in breast cancer and metastatic breast cancer cells. METHOD: Kv1.3 and Kv10.1 channels were inhibited by specific siRNAs using a lipofectamine-based transfection in MCF-7 and MDA-MB-231 cells. After transfection, total RNA was isolated, and then miR-126 and miR-126* expressions were observed using RT-PCR. RESULTS: There was a negative correlation between Kv channels and miRNAs according to the characteristics of the breast cancer cells. The inhibition was observed not only in Kv1.3 but also in Kv10.1 in MCF-7 cells, and miR-126 and miR-126* expressions were downregulated compared to the control group (p < 0.001). The inhibition of these channels in MDA-MB-231 cells caused an upregulation of miR-126 and miR-126* expressions (p < 0.001). CONCLUSION: The miR-126 and miR-126* expressions differed according to benign and malign breast cancer cell lines. Furthermore, we found that miR-126/126* may interact with Kv1.3 and Kv10.1 voltage-gated potassium channels. Our study suggests and indicates the relationship between Kv channels and miRNAs in breast cancer cells (Tab. 1, Fig. 2, Ref. 51). |
||
| Keywords: breast cancer, Kv1.3, Kv10.1, miR-126, miR-126* | ||
| Published online: 22-Jan-2020 | ||
| Year: 2020, Volume: 121, Issue: 2 | Page From: 111, Page To: 116 | |
| doi:10.4149/BLL_2020_014 |
||
|
|
 download file |
|