Home Neoplasma 2020 Neoplasma Vol.67, No.2, p.354–363, 2020

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Neoplasma Vol.67, No.2, p.354–363, 2020

Title: Combined PGE2 with TNF-α promotes laryngeal carcinoma progression by enhancing GRK2 and TRAF2 interaction
Author: Y. F. LI, C. C. HAN, Y. WANG, D. Q. CUI, T. T. LUO, Y. W. ZHANG, Y. MA, W. WEI

Abstract:

TNF-α has been confirmed to promote tumor growth in LSCC. PGE2 expression in LSCC tissues was significantly higher than in tumor-adjacent tissues. In the present work, we aimed to discover the combined role of TNF-α and PGE2 in LSCC progression and its potential mechanisms. TNF-α and PGE2 were quantified by ELISA. TRAF2, MMP-9 and GRK2 expressions were detected by immunohistochemistry and western blot. UM-SCC-11A cell proliferation was tested by CCK-8, and cell migration and invasion were determined by transwell assay. GRK2/TRAF2 interaction was tested by Co-IP. The results showed that TNF-α, PGE2, TRAF2, MMP-9 and GRK2 expressions were significantly higher in tumor tissues than in tumor-adjacent tissues. Higher expressions of TRAF2, MMP-9 and GRK2 were associated with poorer prognosis of LSCC. Combined TNF-α with PGE2 promoted UM-SCC-11A cell proliferation, migration and invasion. The interactions of TRAF2 and GRK2, as well as MMP-9 expression, were upregulated in response to TNF-α and PGE2 co-stimulation. In conclusion, we found crosstalk between PGE2 and TNF-α signaling pathways, and the interaction between GRK2 and TRAF2 led to the activation of TNF-α-TRAF2-MMP-9 signaling and resulted in the progression of LSCC.



Keywords: TNF-α, PGE2, laryngeal squamous cell carcinoma, GRK2, TRAF2, MMP-9
Published online: 21-Mar-2020
Year: 2020, Volume: 67, Issue: 2 Page From: 354, Page To: 363
doi:10.4149/neo_2020_190526N463
Price: 24.00 €






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