Home Acta Virologica 2020 Acta Virologica Vol.64, No.1, p.59-66, 2020

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ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.64, No.1, p.59-66, 2020

Title: Characterization of a P18 protein in the S1 segment of the novel duck reovirus genome
Author: X. DU, M. DING, Q. WU, CH. LI, H. GUO, G. LIU, Z. CHEN

Abstract: Novel duck reovirus (NDRV), the prototype strain of avian orthoreoviruses, continues to circulate among ducks. Analysis of its genome suggested that a putative second open reading frame in the S1 segment encodes a 162-amino acid nonstructural protein with size of 18 kDa, provisionally designated P18. This protein is different from the 17 kDa nonstructural protein encoded in the same open reading frame in other avian orthoreoviruses, which is designated P17 and consists of 146 amino acids. There is no corresponding protein in Muscovy duck reovirus. Antibodies raised to the purified recombinant protein reacted with viral P18 both in vitro and in vivo. In cells, P18 was located predominantly in the nucleus at 6–12 h post-infection, with negligible levels in the cytoplasm. However, the protein accumulated both in the nucleus and cytoplasm at 24 to 36 h post-infection. Immunohistochemistry indicated that P18 strongly accumulates in spleen tissues of infected ducklings. Collectively, the data provide the direct experimental evidence that P18 is expressed by novel duck reovirus both in vivo and in vitro.

Keywords: duck reovirus; expression; characterization; novel P18 protein
Published online: 11-Mar-2020
Year: 2020, Volume: 64, Issue: 1 Page From: 59, Page To: 66
doi:10.4149/av_2020_108


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