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neo_2020_190703N582

Title: MiR-769-5p inhibits cancer progression in oral squamous cell carcinoma by directly targeting JAK1/STAT3 pathway
Author: Y. ZHOU, X. M. XU, Y. FENG

Abstract: Oral squamous cell carcinoma (OSCC) is the most common human malignancy worldwide and a high mortality rate. MiR-769-5p has been reported to be downregulated in tissues and blood of OSCC patients. However, the exact roles and pathogenesis of miR-769-5p involved in OSCC remain unclear. The expressions of miR-769-5p and Janus kinase (JAK1) in OSCC tissue and cells were assessed by RT-qPCR and western blot assay. Expressions of apoptotic-related (Bcl-2, Bax and cleaved-caspase 3) and EMT-associated proteins (MMP9, E-cadherin, N-cadherin, and Vimentin) were detected by western blot assay. The effect of miR-769-5p and JAK1 on proliferation, migration, invasion and apoptosis by CCK-8, transwell and flow cytometry assays, respectively. The binding interaction of miR-769-5p and JAK1 were predicted by Targetscan, and demonstrated by dual-luciferase reporter assays. The volume and weight of the tumour were measured in subcutaneous transplantation experiment. MiR-769-5p was downregulated, and JAK1 was upregulated in OSCC tissue and cells. MiR-769-5p restrained Bcl-2, MMP9, N-cadherin, and Vimentin protein level and accelerated Bax, cleaved-caspase 3 and, E-cadherin protein level, while JAK1 partly overturned these effects. Also, miR-769-5p suppressed proliferation, migration, invasion, and increase apoptosis of OSCC, while the reintroduction of JAK1 abolished these effects. Moreover, JAK1 was verified to be the target of miR-769-5p. In addition, miR-769-5p inhibited the development of OSCC cells in vivo. These results indicate that miR-769-5p suppressed OSCC cells development via targeting JAK1/STAT3 pathway, providing an underlying therapeutic method for OSCC.

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Published online: 17-Feb-2020
Year: , Volume: , Issue: Page From: , Page To:
doi:10.4149/neo_2020_190703N582


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