Home HOME General Physiology and Biophysics 2020 General Physiology and Biophysics Vol.39, No.2, 107–122, 2020

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Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.39, No.2, 107–122, 2020

Title: Solubilisation of model membrane by DDAO surfactant – partitioning, permeabilisation and liposome-micelle transition
Author: Katarína Želinská, Jana Gallová, Silvia Huláková, Daniela Uhríková, Oleksandr Ivankov

Abstract: Solubilisation of model membranes of dioleoylphosphatidylcholine (DOPC) and DOPC-cholesterol (CHOL) induced by surfactant N,N-dimethyl-1-dodecanamine-N-oxide (DDAO) was studied. At the maintained pH ~ 7.5, the DDAO molecules are in their neutral state with respect to the pK ~ 5. Pore formation in lipid bilayer was studied by fluorescence probe leakage method. The changes in the size of lipid aggregates upon increasing DDAO concentration were followed turbidimetrically. Effective ratio Re at different steps of the solubilisation process was determined. The molar partition coefficient of DDAO in case of the DOPC membrane is Kp = 2262 ± 379, for DOPC-CHOL membrane Kp = 2092 ± 594. Within the experimental error, the partition coefficient, as well as effective ratios Re, are not considerably influenced when one third of DOPC molecules is substituted with CHOL (DOPC:CHOL = 2:1). Constituents of buffer (50 mmol/dm3 PBS, 150 mmol/dm3 NaCl) caused aggregation of DOPC and DOPC-CHOL unilamellar liposomes at zero and low DDAO concentration, as was shown by SANS, turbidimetry and DIC microscopy. After solubilisation of bilayer structures by surfactant, mixed DOPC-DDAO and DOPC-CHOL-DDAO micelles with the shape of cylinders with elliptical cross section were detected.

Keywords: N,N-dimethyl-1-dodecanamine-N-oxide — Dioleoylphosphatidylcholine — Cholesterol — Partition coefficient — Solubilisation
Published online: 26-Mar-2020
Year: 2020, Volume: 39, Issue: 2 Page From: 107, Page To: 122

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