Home HOME General Physiology and Biophysics 2020 General Physiology and Biophysics Vol.39, No.2, 195–202, 2020

Journal info

Quarterly, 80 pp. per issue
Founded: 1982
ISSN  1338-4325 (online)

Published in English

Aims and Scope
Editorial Info
Abstracting and Indexing
Submission Guidelines

Select Journal

Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

General Physiology and Biophysics Vol.39, No.2, 195–202, 2020

Title: Lipid-lipid interactions of Escherichia coli mimetic inner membrane at human physiological temperature
Author: Javier Hoyo, Juan Torrent-Burgués,Tzanko Tzanov

Abstract: The current strategies to eradicate bacteria require that the antimicrobial agent either penetrate or disrupt the bacterial membrane. In Escherichia coli (E.coli) as a model of Gram-negative strains, the antimicrobials have to cross two barriers – the outer and the inner membrane being the latter composed by ~ 77% phosphatidylethanolamine (PE), ~ 13% phosphatidylglycerol (PG) and ~ 10% cardiolipin (CL) lipids. Each one of these lipid families shares the same headgroup, but contains acyl chains with varying length and degree of unsaturation. Bacteria adapt their membrane lipid composition and metabolism in response to environmental signals, such as the temperature, resulting in different interactions with exogenous molecules, e.g. antibacterial agents. Herein, bacterial model membranes are prepared to evaluate the lipid-lipid interactions in Langmuir monolayers of binary mixtures at several molar ratios of PE and PG or CL at human physiological temperature (37°C). Both PE:PG and PE:CL monolayers were stable at 37°C and presented higher molecular areas (> 20 Å2/molecule) than at 23°C. However, these lipid mixtures presented liquid-expanded state and rigidity (inverse of the compressibility modulus ~ 90 mN/m) slightly lower than at 23°C. Such athermalicity at biologically relevant temperatures may favour the preservation of the biological functions of E.coli.

Keywords: Phosphatidylethanolamine — Phosphatidylglycerol — Cardiolipin — Lipid-lipid interactions — Bacterial mimetic membranes — Physiological temperature
Published online: 26-Mar-2020
Year: 2020, Volume: 67, Issue: 2 Page From: 195, Page To: 202

download file

© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.