Home Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.4, p.271–277,2020

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.564


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Bratislava Medical Journal Vol.121, No.4, p.271–277,2020

Title: Low concentrations of morphine enhanced the neuroglia-like differentiation
Author: H. Zhaleh, M. Azadbakht, A. Bidmeshki Pour

Abstract: AIM: The present study was undertaken to evaluate the effects of different concentrations of morphine in chronic manner on neuroglial differentiation in PC12 cells.
METHODS: PC12 cells were cultured in RPMI 1640 culture medium including 0.02 % bovine serum albumin together with different concentrations of morphine for 12 days. Cytotoxicity was performed by lactate dehydrogenase assay. Cell death was performed by PI/Hoechst staining assay. Neuroglial differentiation was performed by Nestin, Tuj-1, MAP-2, S-100 and GFAP Immunocytochemistry assay.

RESULTS: Data showed that morphine either at low or high concentration activated opioid  receptors, which  resulted in a decrease of cytotoxicity and cell death and induction of Nestin, Tuj-1, MAP-2, Neurofilament-M (NF-M), GFAP and S-100 protein expression as compared in treated cells  with the control (untreated cells) (p<005).
CONCLUSION: It can be concluded that low concentrations of morphine in chronic manner stimulate the neuroglial-like differentiation by activating protein expression and survival-promoting signaling in PC12 cells with opioid receptor-dependent mechanism (Fig. 8, Ref. 36). Text in PDF www.elis.sk.

Keywords: morphine, naltrexone, neuroglial differentiation, PC12 cell.
Published online: 03-Apr-2020
Year: 2020, Volume: 121, Issue: 4 Page From: 271, Page To: 277

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