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ISSN 1338-4317 (online)

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neo_2020_190625N549

Title: Regulation of HBV replication and gene expression by miR-501-3p via targeting ZEB2 in hepatocellular carcinoma
Author: Z. J. ZHONG, J. F. XU, Z. Z. LI, W. Y. ZHOU, X. X. CHEN, J. H. ZHOU, Z. Y. LI

Abstract: Hepatitis B virus (HBV) infection is a major public health issue with serious medical consequences. The roles of microRNAs (miRNAs) in HBV replication and expression have been generally recognized, and the abnormal expression of miR-501 has been reported in patients with HBV infection. However, the function and mechanism in HBV replication remain elusive. The expression patterns of miR-501-3p and ZEB2 in HBV-related hepatocellular carcinoma (HCC) tissues and HBV-expressing HCC cells were determined by qRT-PCR and western blot assays. HBV replication and expression were evaluated through detecting the copies of HBV DNA and the secretion of HBV surface antigens HBsAg and HBsAg by real-time PCR and ELISA analyses. Bioinformatics and dual-luciferase reporter analyses were employed to validate the functional interaction between miR-501-3p and ZEB2. MiR-501-3p was significantly upregulated, while ZEB2 was downregulated in HBV-related HCC tissues and cells compared with relative controls without HBV infection. Knockdown of miR-501-3p hampered HBV replication and gene expression in HepG2.2.15 and HepAD38 cells. ZEB2 was identified as a functional target of miR-501-3p. Absence of ZEB2 abolished the inhibitory effects of anti-miR-501-3p on HBV replication and gene expression.
Our data indicated that miR-501-3p participated in the regulation of HBV replication and gene expression partially via repressing ZEB2 in HepG2.2.15 and HepAD38 cells, providing a promising antiviral avenue for HBV infection.

Keywords: Hepatitis B virus, miR-501-3p, ZEB2, replication, expression, hepatocellular carcinoma
Published online: 08-May-2020
Year: , Volume: , Issue: Page From: , Page To:
doi:10.4149/neo_2020_190625N549


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