Home HOME General Physiology and Biophysics 2020 General Physiology and Biophysics Vol.39, No.3, p.229–237, 2020

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Quarterly, 80 pp. per issue
Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.39, No.3, p.229–237, 2020

Title: The circRNA-MYLK plays oncogenic roles in the Hep-2 cell line by sponging microRNA-145-5p
Author: Yao Chen, Yanmei Wang, Congcong Li, Xuechang Li, Tiejun Yuan, Shuqin Yang, Xiaoyan Sun

Abstract: For the exploration of circular RNA light chain kinase (circRNA-MYLK), siRNA#1 and siRNA#2 targeting circRNA-MYLK as well as microRNA(miR)-145-5p inhibitor were transfected. Viability was valued with the CCK-8. The protein expression was examined relying on Western blot. The expression of circRNA-MYLK or miR-145-5p was tested depending on qRT-PCR. The apoptotic/migration/invasion rate was separately measured by the Annexin v-FITC/PI with flow cytometer or chambers assays. CircRNA-MYLK was overexpressed in tumor tissue. Silencing circRNA-MYLK induced the inhibitions of viability, invasion and migration, as well as the blocks of MEK/ERK and NF-κB cascades, however, silencing circRNA-MYLK led to provoking of apoptosis. Besides, circRNA-MYLK silencing stimulated the over-production of miR-145-5p, whose silencing abolished the effects of siRNA#1 and siRNA#2 of circRNA-MYLK on those factors above. The circRNA-MYLK had oncogenic roles via targeting miR-145-5p in the Hep-2 cell line via stimulating MEK/ERK and NF-κB cascades.

Keywords: Laryngocarcinoma, circRNA-MYLK, miR-145-5p, MEK/ERK, NF-κB
Published online: 10-Jun-2020
Year: 2020, Volume: 39, Issue: 3 Page From: 229, Page To: 237

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