Home Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.8, p.558–564,2020

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.2

 

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Bratislava Medical Journal Vol.121, No.8, p.558–564,2020

Title: Variation of selected genotoxic and epigenetic markers due to therapeutic exposure to PAHs and ultraviolet radiation
Author: P. Borsky, M. Chmelarova, Z. Fiala, V. Palicka, M. Beranek, J. Kremlacek, C. Andrys, K. Hamakova, A. Malkova, L. Borska

Abstract: BACKGROUND: Goeckerman therapy (GT) of psoriasis involves dermal application of crude coal tar containing polycyclic aromatic hydrocarbons (PAHs) and exposure to ultraviolet radiation (UVR). Little is known about GT influence on DNA epigenetics.
OBJECTIVE: The study aim was to discover epigenetic mechanisms altered by the exposure related to the GT of psoriasis.
METHODS: Observed group of patients with plaque psoriasis (n = 23) was treated by GT with 3 % CCT. Before and after GT, we analyzed the levels of benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA adducts (BPDE-DNA), p53 protein in serum, 5-methylcytosine (5-mC, global DNA methylation), and methylation in selected CpG sites of p53 gene.
RESULTS: We found a significant increase in the levels of BPDE-DNA (p < 0.01) and serum levels of p53 protein (p < 0.01) after GT, and an insignificant decrease in the percentage of 5-mC in peripheral blood DNA. Methylation of p53 CpG sites was affected neither by psoriasis nor by GT. The study confirmed good effectiveness of GT (significantly reduced psoriasis area and severity index; p < 0.001).
CONCLUSION: Our findings indicate that there is a significantly increased genotoxic hazard related to the exposure of PAHs and UV radiation after GT of psoriasis. However, global DNA methylation and p53 gene methylation evade the effect of GT, as they remained unchanged (Tab. 4, Fig. 3, Ref. 50).

Keywords: psoriasis, Goeckerman therapy, methylation, polycyclic aromatic hydrocarbons, UV radiation
Published online: 28-Jul-2020
Year: 2020, Volume: 121, Issue: 8 Page From: 558, Page To: 564
doi:10.4149/BLL_2020_093


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