Home Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.8, p.580–583,2020

Journal info


Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.2


Aims and Scope
Editorial Info
Submission Guidelines

Select Journal

Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Bratislava Medical Journal Vol.121, No.8, p.580–583,2020

Title: Low dosages of vitamin A may cause a decrease in the total neuron number of fetal hippocampal rat cells
Author: H. Ay, D. Aslan, E. Soztutar, F. Yucel

Abstract: OBJECTIVES: We investigated the effect of low, medium and high doses of oral vitamin A, on the number of fetal hippocampal neurons.
BACKGROUND: High doses of vitamin A during pregnancy may cause embryonic malformations. There are reports about dosages that don’t cause macroscopic malformations, but may cause mental and behavioral disorders. Still, quantitative morphological studies explaining this topic are lacking.
METHODS: We administered oral vitamin A to pregnant rats on the 10th–12th days of pregnancy at doses of 10000, 20000, 30000, 40000, 50000, 100000 and 200000 IU/kg. We collected the fetuses on the 19th day and removed their brains. After staining with cresyl violet and immunolabeling with Tunel and Ki67 antibody, we examined the hippocampi with stereological methods.
RESULTS: Vitamin A decreased hippocampal neuron numbers beginning from 20000 IU/kg. While the number of Ki67 positive cells increased with the dosage, the increase of apoptotic cells begun at the dose of 50000 IU/kg.
CONCLUSION: Our study demonstrates that vitamin A, beginning from the dosage of 20000 IU/kg, is decreasing the total hippocampal neuron numbers during the critical period of embryonic brain development and that apoptosis may not be the only factor in this outcome (Tab. 1, Fig. 3, Ref. 27).

Keywords: vitamin A, hippocampus, neuron number, stereology
Published online: 28-Jul-2020
Year: 2020, Volume: 121, Issue: 8 Page From: 580, Page To: 583

download file

© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.