Home Acta Virologica 2020 Acta Virologica Vol.64, No.3, p.331-337, 2020

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ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.64, No.3, p.331-337, 2020

Title: E. coli expression and immunological assessment of expressed recombinant Newcastle disease virus hemagglutinin-neuraminidase protein in chickens
Author: N. Shahid, A. Q. Rao, A. Ahad, A. Gul, A. Latif, S. Azam, M. Shahid, S. Akhtar, A. A. Shahid, T. Husnain

Abstract: Every year, the poultry industry experiences significant economic losses due to epidemics of Newcastle disease virus (NDV). Developing new vaccines by identifying and using the immunogenic hemagglutinin-neuraminidase (HN) protein can protect the poultry industry. In the present study, the full-length HN protein was expressed in Escherichia coli (E. coli) BL21 (DE3) cells, purified via affinity chromatography and detected via western blot analysis using His-specific antibodies. The purified HN protein was further evaluated in chickens to study the immune response against NDV. The successful production of HN-specific IgY proved the activity of the purified HN protein. IgY was present in the serum of immunized chickens. However, the immune response was higher in chickens immunized with purified HN protein along with complete and incomplete adjuvants than in chickens immunized with only the HN protein.

Keywords: protein; Newcastle disease virus; poultry; infectious diseases; vaccines
Published online: 10-Sep-2020
Year: 2020, Volume: 64, Issue: 3 Page From: 331, Page To: 337
doi:10.4149/av_2020_310


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