Home HOME Neoplasma 2021 Neoplasma Vol.68, No.2, p.262–272, 2021

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Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

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Neoplasma Vol.68, No.2, p.262–272, 2021

Title: Upregulation of microRNA-650 by PBX1 is correlated with the development of Helicobacter pylori-associated gastric carcinoma
Author: Junzheng Liu, Lei Wang, Jie Li, Yu Xu

Abstract: Helicobacter pylori (HP) infection induces the development of gastric carcinoma (GC), one of the most frequent and fatal cancers worldwide, via a progressive cascade. The roles of microRNAs (miRNAs) involved in the cascade and the behind mechanisms, however, are still unclear. This study was designed to investigate the expression of miR-650, a well-recognized oncogenic miRNA in GC samples and to analyze the associations between this miRNA and HP infection and the molecular mechanism. Following miRNA- and mRNA-based microarray analyses, miR-650, pre-B-cell leukemia transcription factor 1 (PBX1), and LATS2 were filtered as targets. After that, function assays were implemented to assess their function in GC cells. miR-650 was upregulated in HP+ tissues and cells, and inhibition of miR-650 attenuated cell proliferation, invasion, migration, yet enhanced apoptosis. PBX1 was overexpressed in HP+ tissues and cells and promoted miR-650 transcription. Overexpression of PBX1 abrogated the effect of the miR-650 inhibitor on GC cells. miR-650 targeted LATS2, and LATS2 was poorly expressed in HP+ tissues and cell lines. Simultaneous knockdown of miR-650 and LATS2 reduced GC cell apoptosis. These results display that upregulation of miR-650 induced by HP infection and PBX1 dampens LATS2 in GC cells, potentially offering novel intervention targets for GC.

Keywords: Helicobacter pylori; microRNA-650; Gastric carcinoma; PBX1; LATS2
Published online: 04-Nov-2020
Year: 2021, Volume: 68, Issue: 2 Page From: 262, Page To: 272

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