Home Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.11, p.805–811,2020

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.2

 

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Bratislava Medical Journal Vol.121, No.11, p.805–811,2020

Title: Caffeic acid suppresses HT-29 cell death induced by H2O2 via oxidative stress and apoptosis
Author: E. Darendelioglu

Abstract: AIM: The purpose of this research was to examine the suppressing effect of caffeic acid (CA) on colon cancer cells triggered by an overdose of H2O2, and molecular mechanisms involved.
METHOD: This study examines cell proliferation, measurement of ROS and lipid peroxidation (LPO) levels, total antioxidant status (TAS) level, catalase (CAT) activity, TUNEL assay for calculating the apoptotic index, immunohistochemical staining for caspase-3 proteins, and qRT-PCR for measuring mRNA levels of apoptotic and anti-apoptotic genes.
RESULTS: In this study, CA considerably suppressed HT-29 cell death induced by cytotoxicity achieved by and overdose of H2O2. Additionally, inducing cells with H2O2 caused a rise in ROS and LPO levels, decrease in TAS level and CAT activity whereas pre-treatment of cells with CA reversed these effects. Additionally, a considerable increase was observed in the expression of Bax, cas-3, cas-8, cyt c, p53 at mRNA levels after H2O2 treatment, however, pre-treatment with CA considerably decreased H2O2-induced upregulation of these genes.
CONCLUSION: In light of all these findings, the antioxidant use should be paid attention to as it could decrease the level of ROS, and in turn decrease the apoptotic cell death which is an unwanted situation in the setting of cancer remedy. Overall, these data revealed that CA can suppress apoptosis in HT-29 cells triggered by an overdose of H2O2 (Fig. 5, Ref. 37).

Keywords: oxidative stress, HT-29, caffeic acid, cyt c, cas-8; apoptosis
Published online: 09-Nov-2020
Year: 2020, Volume: 121, Issue: 11 Page From: 805, Page To: 811
doi:10.4149/BLL_2020_132


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