Home 
FOR AUTHORS Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.12, p.853–857,2020
FOR AUTHORS Bratislava Medical Journal 2020 Bratislava Medical Journal Vol.121, No.12, p.853–857,2020
Journal info
|
||||
Select Journal
Journals
Bratislava Medical Journal 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 Endocrine Regulations General Physiology and Biophysics Neoplasma Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Bratislava Medical Journal Vol.121, No.12, p.853–857,2020 |
||
| Title: The effect of growth factors on induction of mesenchymal stem cell to the skeletal muscle cells | ||
| Author: B. Dogan, D. Kaan, H. Ulger | ||
| Abstract: OBJECTIVES: The objective of this study was to evaluate the effect of IGF1 and FGF2 growth factors on the differentiation of human adipose tissue‑derived mesenchymal stem cells (MSCs) into skeletal muscle cells. METHODS: MSCs were divided into four groups. Group I (control group) was incubated only in myogenic medium, group II was incubated by adding 100 ng/ml FGF2 to the myogenic medium, group III was incubated by adding 20 ng/ml IGF1 to the myogenic medium, group IV was incubated by adding 100 ng/ml FGF2 and 20 ng/ml IGF1 to the myogenic medium. Cells dyed with MyoD1 antibodies were analyzed by flow cytometry so as to determine the myogenic differentiation on day 28. RESULTS: It was confirmed that 11.5 % of the control group, 39.2 % of the FGF2 group, 23.1 % of the IGF1 group, and 39.3 % of the IGF1-FGF2 group showed differentiation. CONCLUSION: Our results show that IGF1 and FGF2 have a positive contribution to myogenic differentiation of MSCs. They contribute to the studies related to muscle diseases and their treatment by the fact that growth factors support the feature of regeneration capacity and differentiation (Tab. 2, Fig. 7, Ref. 23). |
||
| Keywords: stem cell, myogenic differentiation, IGF, FGF | ||
| Published online: 09-Dec-2020 | ||
| Year: 2020, Volume: 121, Issue: 12 | Page From: 853, Page To: 857 | |
| doi:10.4149/BLL_2020_140 |
||
|
|
 download file |
|