Home Bratislava Medical Journal 2021 Bratislava Medical Journal Vol.122, No.9, p.621–625,2021

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.564

 

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Bratislava Medical Journal Vol.122, No.9, p.621–625,2021

Title: Recovery potential of transplanted oligoprogenitor cells derived from human dental pulp stem cells in Lysophosphatidyl choline demyelination mode
Author: H. G. Hamidabadi, M. N. Bojnordi, S. Ehsani
Abstract: IM: Here we used a demyelination model using an injection of Lysophosphatidylcholine )LPC( in the corpus collosum to examine the myelination activity of differentiated oligodendrocytes derived from Human dental pulp stem cells )hDPDSCs( according to a two step induction protocol.
METHODS AND MATERIALS: The cells were cultured in DMEM-F12 medium containing 1M Retinoic acid and were treated with 5ng/ml platelet-derived growth factor, 10 ng/ml basic fibroblast growth factor for 8-10 days. The differentiation cells were examined via the expression of specific glial markers: Olig2 and O4. Cells were transplanted in to a demylinated rat corpus callosum. The alteration of the demyelination extension as well as remyelination intensity was examined via a specific myelin staining: Luxol Fast Blue and immunohistochemistry.
RESULTS: Differentiated oligoprogenitor cells were confirmed via immunofluorescence staining with specific glial markers: Olig2 and O4. Also, the amount of demyelination was decreased and intensity of remyelination showed an increase after an engraftment of differentiated cells. Immunohistochemistry for evaluation of PLP expression proved the mature myelinating oligodendrocytes.
CONCLUSION: hDPSCs can be induced to transdifferentiate into oligoprogenitor cells and respond to the routinely applied regents for glial differentiation of Mesenchymal stem cells. hDPSCs may be a valuable source for cell therapy in neurodegenerative diseases (Fig. 4, Ref. 30). Text in PDF www.elis.sk
Keywords: dental pulp stem cells, lysophosphatidylcholine, corpus callosum, oligodendrocyte, differentiation
Published online: 31-Aug-2021
Year: 2021, Volume: 122, Issue: 9 Page From: 621, Page To: 625
doi:10.4149/BLL_2021_099


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