Home Bratislava Medical Journal 2021 Bratislava Medical Journal Vol.122, No.10, p.732–738,2021

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.2

 

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Bratislava Medical Journal Vol.122, No.10, p.732–738,2021

Title: Potential benefits of using hydrogen sulfide, vitamin E and necrostatin-1 to counteract acetaminophen‑induced hepatotoxicity in rats
Author: N. E. H. Saleh, A. H. Saad, E. A. Elbassuoni, N. F. El-Tahawy, E. A. Abdel-Hakeem

Abstract: BACKGROUND: The use of acetaminophen (APAP) is increasing recently, especially with COVID-19 outbreaks. APAP is safe at therapeutic levels, however, an overdose can cause severe liver injury. This study aims to explore possible mechanisms involved in APAP‑induced hepatotoxicity and compare different hepatoprotective agents, namely vitamin E, hydrogen sulfide (H2S) and necrostatin-1 (NEC-1).
METHODS: Adult male albino rats were divided into groups: Control group, APAP‑induced hepatotoxicity group, Vitamin E‑treated group, H2S‑treated group and NEC-1‑treated group. Serum levels for aspartate aminotransferase (AST), alanine aminotransferase (ALT), interleukin-33 (IL-33), tumor necrosis factor alpha (TNF-α), reduced glutathione (GSH) and lipid profile were measured. Histopathological examinations of liver tissue with H(et)E stain and immunohistochemistry for activated caspase-3 were also done.
RESULTS: APAP‑treated group showed elevated liver transaminases, hyperlipidemia, and deficient liver anti-oxidative response together with disturbed hepatic architecture and increased immune-expression of activated caspase-3 in hepatic tissue. Pretreatment with vitamin E, H2S or NEC-1 reversed the affected parameters. Vitamin E and H2S showed greater improvement when compared to NEC-1.
CONCLUSION: Vitamin E, H2S and NEC-1 showed protective effects against APAP-induced hepatotoxicity, thus they may be used as an adjuvant therapy when APAP is indicated for long periods as is the case in COVID-19 patients (Tab. 2, Fig. 2, Ref. 45). Text in PDF www.elis.sk

Keywords: acetaminophen, hepatotoxicity, apoptosis, necrostatin-1, vitamin E, H2S
Published online: 27-Sep-2021
Year: 2021, Volume: 122, Issue: 10 Page From: 732, Page To: 738
doi:10.4149/BLL_2021_117


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