Journal info
|
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics Neoplasma 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Neoplasma Vol.69, No.1, p.49–58, 2022 |
||
Title: SNHG16 lncRNAs are overexpressed and may be oncogenic in human gastric cancer by regulating cell cycle progression | ||
Author: Juan-Juan Zhao, Juan-Juan Liu, Yun-Yuan Zhang, Ying Xia, Hong Du, Zhi-Qiang Yan, Chun-Huan Zhou, Wan-Song Xia, Lucas Zellmer, De-Zhong Joshua Liao, Si-Xi Wei, Hai Huang | ||
Abstract: The small nucleolar RNA host gene 16 (SNHG16) has recently been shown to be a putative oncogene in gastric cancer (GC) and other cancer types, but how its four lncRNA variants are expressed in any physiological and pathological situation remains unknown. To investigate the expression and function of the four lncRNA variants of SNHG16, mainly the variant 1, in GC, we performed quantitative PCR to determine the RNA levels of the four variants in 60 GC tissue samples and several cell lines. We also studied how knocking down of SNHG16 with siRNA affected proliferation, apoptosis, cell cycle progression, as well as migration and invasion of GC cells. Our results showed that variants 1 and 4 were overexpressed in GC tissues compared with adjacent uninvolved tissues. Knockdown of the four variants, mainly the variant 1, enhanced apoptosis and inhibited cell cycle progression of a GC cell line by arresting the cells at the G1 phase. These cellular effects were associated not only with decreased protein levels of c-Myc, PCNA, cyclins D, E, A, and B, as well as CDKs 2 and 6, but also with increased protein levels of the p21, p27, and p53. Knockdown of total SNHG16 lncRNAs also inhibited invasion and migration of the GC cells in vitro. These results collectively suggest that SNHG16 may be oncogenic in GC by regulating cell cycle progression and may serve as a GC biomarker. |
||
Keywords: SNHG16, LncRNA variant, gastric cancer, tumor progression, biomarker, oncogenic | ||
Published online: 06-Dec-2021 | ||
Year: 2022, Volume: 69, Issue: 1 | Page From: 49, Page To: 58 | |
doi:10.4149/neo_2021_210518N680 |
||
|
download file |
|