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HOME General Physiology and Biophysics 2022 General Physiology and Biophysics Vol.41, No.1, p. 71–78, 2022
HOME General Physiology and Biophysics 2022 General Physiology and Biophysics Vol.41, No.1, p. 71–78, 2022
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General Physiology and Biophysics Vol.41, No.1, p. 71–78, 2022 |
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| Title: FAM134B-mediated ER-phagy alleviates endoplasmic reticulum stress of rat soleus muscle in response to acute exercise | ||
| Author: Songlin Jin, Lunyu Li, Zaifang Ren, Junping Li, Ruiyuan Wang, Haili Ding | ||
| Abstract: ER-phagy is a selective endoplasmic reticulum (ER) autophagy mediated by ER-localized receptors, which ensures proper cellular homeostasis under stress. However, it remains unclear whether ER-phagy is involved in skeletal muscle response to exercise stress. Male 8-week-old Sprague-Dawley rats were subjected to an exercise protocol comprising a 90-min downhill run with a slope of −16° and a speed of 16 m/min. The soleus of the rats was sampled at 0, 12, 24, 48, and 72 h after exercise. After exercise, the sarcoplasmic/ER calcium ATPase (SERCA) content decreased, the protein disulphide isomerase (PDI) content increased, and ER stress (GRP78 and CRT) and autophagy (FAM134B and LC3)-related protein expression increased in the soleus muscle of rats, and gradually recovered with time. We also used pharmacological methods to simulate the effects of exercise stress on skeletal muscle cells to further explore the mechanism of ER-phagy in skeletal muscle cells. Thapsigargin was used to inhibit the SERCA pump of L6 myoblasts and successfully induce ER stress and activate ER-phagy. During this process, the ER-phagy receptor FAM134B anchors and fragments ER, and then binds with LC3 to form autophagosomes. These results suggest that ER-phagy is involved in the skeletal muscle cell response to exercise stress, which helps to maintain cellular ER homeostasis during exercise. |
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| Keywords: Endoplasmic reticulum stress — ER-phagy — Skeletal muscle — Acute exercise | ||
| Published online: 14-Feb-2022 | ||
| Year: 2022, Volume: 41, Issue: 1 | Page From: 71, Page To: 78 | |
| doi:10.4149/gpb_2021046 |
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