Neoplasma Vol.69, No.3, p.560–570, 2022
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| Title: sh-HNF1A-AS1 reduces the epithelial-mesenchymal transition and stemness of esophageal cancer cells |
| Author: Zheng Wang, Yan-Feng Huang, Ling Yu, Yue Jiao |
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Abstract: HNF1A-AS1 is a cancer-related lncRNA. Elevated expression of HNF1A-AS1 has been observed in various malignancies including esophageal squamous cell carcinoma (ESCC). However, the role of HNF1A-AS1 in ESCC has not been fully understood. This study aimed to investigate the potential role of HNF1A-AS1 in ESCC. Expression of HNF1A-AS1, miRNA (miR)-298, and transcription factor 4 (TCF4) was detected using qRT-PCR. The interactions between HNF1A-AS1 and miR-298 or miR-298 and TCF4 were evaluated. Short hairpin RNAs (shRNAs) were used to knock down HNF1A-AS1 (sh-HNF1A-AS1). The EMT and stemness of ESCC cells were detected. HNF1A-AS1 was overexpressed in ESCC tumor tissues and cells. miR-298 was validated as a direct target of HNF1A-AS1. sh-HNF1A-AS1 significantly inhibited EMT and stemness of ESCC cells. The high expression of miR-298 significantly inhibited the expression of TCF4, and further inhibited the expression of N-cadherin and stemness-related genes. Animal experiments showed that sh-HNF1A-AS1 significantly inhibited tumor growth and increased the level of miR-298 in tissues. In conclusion, knockdown of HNF1A-AS1 could inhibit EMT and stemness by regulating the miR-298/TCF4 axis.
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| Keywords: HNF1A-AS1; miR-298; esophageal squamous cell carcinoma; epithelial-mesenchymal transition; stemness; transcription factor 4 |
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Published online: 21-Feb-2022
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| Year: 2022, Volume: 69, Issue: 3 |
Page From: 560, Page To: 570 |
doi:10.4149/neo_2022_211102N1555
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