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FOR AUTHORS General Physiology and Biophysics 2022 General Physiology and Biophysics Vol.41, No.2, p. 133–140, 2022
FOR AUTHORS General Physiology and Biophysics 2022 General Physiology and Biophysics Vol.41, No.2, p. 133–140, 2022
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General Physiology and Biophysics Vol.41, No.2, p. 133–140, 2022 |
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| Title: Junctophilin-2 allosterically interacts with ryanodine receptor type 2 to regulate calcium release units in mouse cardiomyocytes | ||
| Author: Tianxia Luo, Ningning Yan, Mengru Xu, Fengjuan Dong, Qian Liang, Ying Xing, Hongkun Fan | ||
| Abstract: The interaction between junctophilin-2 (JPH2) and ryanodine receptor type 2 (RyR2) regulated Ca2+ signaling in mouse cardiomyocytes. However, their exact interaction remains unclear. This study elucidates the interaction between JPH2 with RyR2 using co-immunoprecipitation of cardiac sarcoplasmic reticulum vesicles. Additionally, a glutathione S-transferase (GST) pull-down analysis was performed to investigate the physical interaction between RyR2 and JPH2 fragments. JPH2 interacted with RyR2 and the C terminus of the JPH2 protein can pull-down RyR2 receptors. Confocal immunofluorescence imaging indicated that the majority of JPH2 and RyR2 proteins were colocalized near Z-lines in isolated mouse cardiomyocytes. Knockdown of JPH2 reduced the amplitude of Ca2+ transients and disrupted its interaction with RyR2. Therefore, the C-terminus domain of JPH2 is required for interactions with RyR2 in mouse cardiomyocytes, which provides a molecular mechanism for seeking Ca2+-related disease prevention strategies. |
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| Keywords: unctophilin-2 — Ryanodine receptor type 2 — Cardiomyocytes — Ca2+ release | ||
| Published online: 11-Apr-2022 | ||
| Year: 2022, Volume: 41, Issue: 2 | Page From: 133, Page To: 140 | |
| doi:10.4149/gpb_2022007 |
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