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CONTACT Acta Virologica 2022 Acta Virologica Vol.66, No.2, p. 186 – 191, 2022
CONTACT Acta Virologica 2022 Acta Virologica Vol.66, No.2, p. 186 – 191, 2022
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Acta Virologica Vol.66, No.2, p. 186 – 191, 2022 |
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| Title: Rapid detection of sweepoviruses through lateral flow dipstick-based recombinase polymerase amplification | ||
| Author: He Wang, Xiukun Yang, Decai Tuo, Yonghua Liu, Peng Zhou, Wentao Shen, Guopeng Zhu | ||
| Abstract: Sweepoviruses represent a phylogenetic group of begomoviruses that cause significant sweet potato (Ipomoea batatas) production losses in various countries across the world. For rapid identification of sweepoviruses, we developed a technique based on isothermal recombinase polymerase amplification in conjunction with lateral flow dipsticks (RPA-LFD). The optimum reaction conditions for the RPA were 20 min incubation at 37°C. The RPA-LFD specifically detected distinct sweepovirus species, with no other viruses infecting sweet potato causing a cross-reaction. The detection limit of the RPA-LFD was 1.0×104 copies of the target DNA molecule per reaction, and it exhibited a 10-fold greater sensitivity than the conventional PCR. Furthermore, when coupled with an alkaline polyethylene glycol-based crude genomic DNA extraction, the entire procedure was completed in 30 min without the use of any special instruments other than a water bath. Therefore, the RPA-LFD technique is a potential sweepovirus diagnostic tool that can be used in the field with fewer available resources. |
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| Keywords: detection; sweepoviruses; recombinase polymerase amplification; lateral flow dipstick | ||
| Published online: 29-Jun-2022 | ||
| Year: 2022, Volume: 66, Issue: 2 | Page From: 186, Page To: 191 | |
| doi:10.4149/av_2022_208 |
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