Home Bratislava Medical Journal 2022 Bratislava Medical Journal Vol.123, No.12, p.878–884, 2022

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.564


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Bratislava Medical Journal Vol.123, No.12, p.878–884, 2022

Title: Protective effect of piperlongumine on inflammation and oxidative stress against ischemia-reperfusion injury in animal kidney
Author: Zhenwen XIAO, Annamalai VIJAYALAKSHMI

Abstract: AIM: Piperlongumine (PL), an alkaloid from the Piper longum plant, is acknowledged for various biological properties. The study aimed to explore the protective effect of PL on ischemia reperfusion injury (I/R) in rat kidney.
METHODS. 24 adult male Sprague-Dawley rats (200 to 250 mg) were randomly allocated to four groups (n = 6/group): Group I: sham control, Group II: (I/R) renal ischemia/reperfusion kidney renal blocked for 1hour using clamps, followed by 2hr reperfusion. Group III: PL (25 μg/kg) + I/R group and Group IV: PL (50 μg/kg) + I/R group. Rat kidneys were exposed to 60 min of two-sided deep ischemia followed by 120 min of reperfusion. PL (25 and 50 μg/kg bw) was administered intraperitoneally half an hour before the ischemia. Creatinine, urea, and few renal markers activity in serum were assessed. Oxidative stress and inflammatory markers were also evaluated. In addition, the expressions of COX-2 and eNOS in animal kidneys were tested by western blotting.
RESULTS. Pre-treatment with PL in ischemia‑reperfused rats significantly reduced the pathological damage in the kidney and declined the levels of serum creatinine and other renal parameters. PL treatment diminished the serum levels of TNF-α, IL-6, and IL-1β, as well as messenger RNA expressions. Important biological defence parameters such as superoxide dismutase and glutathione levels were upregulated while malondialdehyde levels were down-regulated in PL ischemia rats.
CONCLUSION. PL exhibits a protective effect against inflammation and oxidation in ischemia reperfusion animals (Fig. 5, Ref. 32).

Keywords: piperlongumine, ischemia reperfusion, inflammation, oxidative stress
Published online: 20-Oct-2022
Year: 2022, Volume: 123, Issue: 12 Page From: 878, Page To: 884

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