Acta Virologica Vol.66, No.4, p. 301

Acta Virologica Vol.66, No.4, p. 301 – 308, 2022

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Acta Virologica Vol.66, No.4, p. 301 – 308, 2022
Title: Integrase deletion mutation impairs the Gag cleavage in prototype foamy virus
Author: Jinsun Kim, Cha-Gyun Shin

Abstract: Integrase (IN) is an essential enzyme that catalyzes integration of viral DNA into host cellular genome. Among retroviruses, foamy virus is the first in which the X-ray crystal structure of IN has been reported. Compared with other retroviruses, this virus has a unique expression pattern since Gag and Pol are produced independently from each other. For the successful maturation of prototype foamy virus (PFV), Gag should be cleaved into a 68/71-kDa Gag doublet. Here, we induced deletion mutations in PFV IN and characterized their effects on the Gag cleavage. Among the generated serial mutants, only those with deletions of up to two amino acids showed the wild-type Gag-cleavage pattern and viral titer level. These results were confirmed via transmission electron microscopy and site-specific mutagenesis. Taking together, maturation and morphogenesis, the deletion mutations of more than two amino acids in catalytic core domain (CCD) region are able to compromise the viral reproduction.

Keywords: integrase; prototype foamy virus; deletion mutant; gag cleavage
	Published online: 09-Dec-2022
Year: 2022, Volume: 66, Issue: 4	Page From: 301, Page To: 308
doi:10.4149/av_2022_401
	download file

Aepress.sk