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Bratislava Medical Journal 2023 Bratislava Medical Journal Vol.124, No.2, p.143–150, 2023
Bratislava Medical Journal 2023 Bratislava Medical Journal Vol.124, No.2, p.143–150, 2023
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Bratislava Medical Journal Vol.124, No.2, p.143–150, 2023 |
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| Title: Silencing of Long noncoding RNA SOX2-OT relieves myocardial ischemia/reperfusion injury through up-regulating microRNA-146a-5p | ||
| Author: Zhongxin LI, Guangdong LIU, Hua HUANG | ||
| Abstract: PURPOSE: Long noncoding RNAs (lncRNAs) are involved in the development of myocardial ischemia/reperfusion injury (MIRI). In this study, we aimed to explore the regulatory effect and mechanism of lncRNA SOX2-OT in MIRI. METHODS: The expression levels of SOX2-OT and miR-146a-5p in OGD/R-treated H9C2 cells and in myocardial tissues of MIRI rats were measured by qRT-PCR. Cell viability was detected by MTT assay. The levels of IL-1β, IL-6, TNF-α, MDA, and SOD were measured by ELISA. The target relationship between SOX2-OT and miR-146a-5p was predicted by LncBase, and subsequently confirmed by DLR assay. The effects of SOX2-OT silencing on myocardial apoptosis and function were further validated in MIRI rats. RESULTS: The expression of SOX2-OT was increased in OGD/R-treated H9C2 cells and myocardial tissues of MIRI rats. Silencing of SOX2-OT increased the viability and inhibited the inflammation and oxidative stress of OGD/R-treated H9C2 cells. SOX2-OT negatively regulated its target miR-146a-5p. Inhibition of miR-146a-5p reversed the effects of sh-SOX2-OT on increasing the viability, and on inhibiting the inflammation and oxidative stress of OGD/R-treated H9C2 cells. In addition, silencing of SOX2-OT alleviated myocardial apoptosis and improved myocardial function in MIRI rats. CONCLUSIONS: Silencing of SOX2-OT relieved the apoptosis, inflammation, and oxidative stress of myocardial cells via up-regulating miR-146a-5p, contributing to the remission of MIRI (Fig. 28, Ref. 33). |
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| Keywords: SOX2-OT, miR-146a-5p, myocardial ischemia/reperfusion injury, inflammation | ||
| Published online: 03-Jan-2023 | ||
| Year: 2023, Volume: 124, Issue: 2 | Page From: 143, Page To: 150 | |
| doi:10.4149/BLL_2023_022 |
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