Journal info
|
||||
Select Journal
Journals
Bratislava Medical Journal 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 Endocrine Regulations General Physiology and Biophysics Neoplasma Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Bratislava Medical Journal Vol.124, No.6, p.437–441, 2023 |
||
Title: Astroglial differentiation of fibronectin‑positive human “glia-like” cells | ||
Author: Ivana SIVAKOVA, Peter MRAZ, Anna PERZELOVA | ||
Abstract: OBJECTIVES: Fibronectin (Fn) is a glycoprotein of extracellular matrix produced by a variety of mesenchymal and neoplastic cell types. BACKGROUND: In adult brain tissue, Fn is restricted to blood vessels. However, adult human brain cultures are almost entirely comprised of flat or spindle‑shaped Fn-positive cells usually referred to as “glia-like” cells. Since Fn is primarily present in fibroblasts, these cultures may be considered to be of non-glial origin. METHODS: Cells gained by long-term culturing of adult human brain tissue derived from brain biopsies obtained from 12 patients with non-malignant diagnoses were examined by immunofluorescence methods. RESULTS: Primary cultures contained GFAP–/Vim+/Fn+ “glia-like” cells (95–98 %) and GFAP+/Vim+/Fn- astrocytes (0.1 %) which disappeared by passage number 3. The formation of cell processes and enlargement of cell bodies was observed in 9 of 12 cultures with decreased cell growth during passages 12 to 17. It is remarkable that during this period, all “glia-like” cells became GFAP+/Vim+/Fn+. CONCLUSION: Herein, we confirm our previously published hypothesis about the origin of adult human “glia-like” cells, which we consider to be precursor cells scattered through the brain cortex and subcortical white matter. Cultures were comprised entirely of GFAP–/Fn+ “glia-like” cells and showed morphological and immunochemical astroglial differentiation with spontaneously decelerated growth during prolonged passaging. We propose that the adult human brain tissue contains a “dormant population” of undefined glial precursor cells. Under culture, these cells show to have a high proliferative capacity and different stages of cell dedifferentiation (Fig. 2, Ref. 21). |
||
Keywords: astrocytes, GFAP, fibronectin, glial differentiation, glia-like cells | ||
Published online: 28-Feb-2023 | ||
Year: 2023, Volume: 124, Issue: 6 | Page From: 437, Page To: 441 | |
doi:10.4149/BLL_2023_066 |
||
|
![]() |
|