Neoplasma Vol.70, No.2, p. 216–228, 2023
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| Title: Hypoxia-induced miR-9 expression promotes ovarian cancer progression via activating PI3K/AKT/mTOR/GSK3β signaling pathway |
| Author: Wen-Jing Zhu, Huan-Huan Huang, Yi-Fan Feng, Lei Zhan, Jian Yang, Lin Zhu, Qing-Yuan Wang, Bing Wei, Wen-Yan Wang |
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Abstract: Ovarian cancer (OC) is one of the most prevalent malignant tumors affecting women’s life and health. Since OC has a poor prognosis due to extensive metastasis, there is a need to explore a new mechanism of OC metastasis. microRNAs (miR) are single-stranded, non-coding RNAs. miR-9 has been reported to promote cancer and may provide a new strategy for OC diagnosis. The purpose of this study was to examine the function and underlying mechanism of miR-9 in OC. RT-qPCR was used to assess miR-9 expression levels. Transwell assays were used to determine the number of migrating and invading OC cells. The protein expression levels of the PI3K/AKT/mTOR/GSK3β signaling pathway were examined using western blotting. The results informed that, when compared to normal ovarian tissues, miR-9 was remarkably expressed in OC tissues, and hypoxia might lead to overexpression of miR-9-5p while inhibiting miR-9 notably suppressed the migrating and invading cell numbers in OC cells. In vivo, miR-9-5p knockdown inhibited tumor growth in a subcutaneous nude mice model of SKOV3 cells. Our findings suggest that miR-9 could be an underlying oncogene in OC, opening up new avenues for OC diagnosis and treatment of OC by targeting miR-9.
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| Keywords: miR-9; hypoxia; ovarian cancer; migration and invasion; PI3K/AKT/mTOR/GSK3β |
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Published online: 25-Mar-2023
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| Year: 2023, Volume: 70, Issue: 2 |
Page From: 216, Page To: 228 |
doi:10.4149/neo_2023_221103N1079
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