Home General Physiology and Biophysics 2023 General Physiology and Biophysics Vol.42, No.4, p. 339–347, 2023

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Founded: 1982
ISSN 1338-4325 (online)
ISSN 0231-5882 (print)
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General Physiology and Biophysics Vol.42, No.4, p. 339–347, 2023

Title: A diagnostic model based on gene biomarkers for Crohn’s disease
Author: Shasha Wu, Lin Zeng, Jisheng Wang
Abstract: Crohn’s disease (CD) is a segmental chronic inflammatory bowel disease, which seriously affects the patient’s quality of life. The etiology of CD is not yet clear, and there is still a lack of effective treatments. Therefore, in this study, we focus on developing a useful model for early diagnosis and targeted therapy of CD. The expression datasets of CD were collected to filter differentially expressed genes (DEGs) by overlapping “limma” package and “WGCNA” package. Then, functional enrichment analysis and protein-protein interaction (PPI) network analyses were performed. Hub genes were screened with “cytoHubba” plug-in and filtered with LASSO and stepwise regression analyses. The logistic regression model and nomogram were established based on the selected hub genes. The 45 DEGs were identified and the top 30 hub genes were chosen out for further study. Finally, 11 genes were selected to construct the logistic regression model and nomogram. The receiver operating characteristic (ROC) curve shows that the area under the curve (AUC) value was 0.960 in the training dataset and 0.760 in the validation dataset. A 11-gene diagnostic model was constructed with IL1B, CXCL10, CXCL2, LCN2, MMP12, CXCL9, NOS2, GBP5, FPR1, GBP4 and WARS, which may become potential biomarkers for early diagnosis and targeted therapy of CD.
Keywords: Crohn’s disease — Diagnosis — Logistic model — Nomogram — Biomarker
Published online: 03-Jul-2023
Year: 2023, Volume: 42, Issue: 4 Page From: 339, Page To: 347
doi:10.4149/gpb_2023012


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