Home General Physiology and Biophysics 2024 General Physiology and Biophysics Vol.43, No.4, p. 353–366, 2024

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Founded: 1982
ISSN 1338-4325 (online)
ISSN 0231-5882 (print)
Published in English,
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General Physiology and Biophysics Vol.43, No.4, p. 353–366, 2024

Title: Berberine protects against sepsis-related acute lung injury in rats via PPAR-γ signaling pathway upregulation and improvement at the cellular level: Functional, biochemical, and immunohistochemistry study
Author: Mohamed M. Khalifa, Nermeen A. Bastawy, Laila A. Rashed, Hanan A. Hassan, Omnia M. Abdel-Maksoudand Fatma E. Hassan
Abstract: This study aimed to assess the prophylactic effects of Berberine on experimentally induced lung sepsis and examine its effects on selected cytokines, genes, and protein expression besides the histopathological evaluation. Berberine significantly reduced the wet/dry lung ratio, the broncho-alveolar lavage fluid (BALF) protein, cells, neutrophils percentage, and cytokines levels. In addition, pretreatment with Berberine decreased the myeloperoxidase (MPO) and malondialdehyde (MDA) levels and decreased gene expression of nuclear factor kappa B (NF-κB), monocyte chemoattractant protein-1 (MCP-1), and the intracellular adhesion molecule 1 (ICAM-1) by RT-qPCR analysis, revealing Berberine’s antioxidant and anti-inflammatory mode of action. Western blot analysis revealed increased peroxisome proliferator-activated receptor gamma (PPAR-γ) expression in the Berberine pretreated group compared to the cecal ligation and puncture (CLP) group, in which the histopathological examination evidenced this improvement. In conclusion, Berberine improved lung sepsis via its PPAR-γ mediated antioxidant and anti-inflammatory effects.
Keywords: Berberine — Septic lung injury — CLP — Peroxisome proliferator-activated receptor gamma (PPAR-γ)
Published online: 29-Jun-2024
Year: 2024, Volume: 43, Issue: 4 Page From: 353, Page To: 366
doi:10.4149/gpb_2024008


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