Home General Physiology and Biophysics 2024 General Physiology and Biophysics Vol.43, No.5, p, 411–421, 2024

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General Physiology and Biophysics Vol.43, No.5, p, 411–421, 2024

Title: Protective role of S-adenosylmethionine on high fat/high cholesterol diet-induced hepatic and aortic lesions and oxidative stress in guinea pigs
Author: İlknur Bingül, Canan Küçükgergin, Abdurrahman Fatih Aydın, Aydın Çevik, Merva Soluk-Tekkeşin, Vakur Olgaç, Semra Doğru-Abbasoğlu, Müjdat Uysal
Abstract: S-adenosylmethionine (SAM) is the main methyl group donor and has antioxidant potential. In this study, preventive and regressive potential of SAM were investigated in high fat/high cholesterol (HFHC) diet-induced non-alcoholic fatty liver disease (NAFLD) in guinea pigs. They were injected with SAM (50 mg/kg, i.p.) for 6 weeks along with HFHC diet or 4 weeks after HFHC diet. Serum transaminase activities, total cholesterol (TC), triglyceride (TG), cytochrome p450-2E1 (CYP2E1) and hydroxyproline (Hyp) levels, prooxidative and antioxidative parameters, protein expressions of α-smooth muscle actin (α-SMA) and transforming growth factor-β1 (TGF-β1) together with histopathological changes were examined in the liver. SAM treatment diminished HFHC diet-induced increases in serum transaminase activities and hepatic TC, TG, CYP2E1, Hyp, α-SMA and TGF-β1 expressions and ameliorated prooxidant-antioxidant balance. Histopathological scores for hepatic steatosis, inflammation, and fibrosis were decreased by SAM treatment. Increases in TC, diene conjugate levels, and lipid vacuoles within the tunica media of the aorta were reduced in HFHC-fed animals treated with SAM. These protective effects were also detected in the regression period of HFHC-guinea pigs due to SAM. In conclusion, SAM treatment was found to be effective in prevention and regression of HFHC-induced hepatic and aortic lesions together with decreases in oxidative stress in guinea pigs with NAFLD.
Keywords: S-adenosylmethionine — Non-alcoholic steatohepatitis (NASH) — High fat/high cholesterol diet — Oxidative stress — Guinea pigs
Published online: 16-Aug-2024
Year: 2024, Volume: 43, Issue: 5 Page From: 411, Page To: 421
doi:10.4149/gpb_2024021


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