Acta Virologica Vol.49, p.243-250, 2005
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Title: DIFFERENCES IN ANTIBODY RESPONSES OF MICE TO INTRANASAL
OR INTRAPERITONEAL IMMUNIZATION WITH INFLUENZA A VIRUS
AND VACCINATION WITH SUBUNIT INFLUENZA VACCINE |
Author: T. FISLOVÁ, T. SLÁDKOVÁ, M. GOCNÍK, V. MUCHA, E. VAREČKOVÁ, F. KOSTOLANSKÝ |
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Abstract: Two antigenically related but different influenza A virus strains of H3N2 subtype, A/Dunedin/
4/73 (H3N2) (Dunedin) and A/Mississippi/1/85 (H3N2) (Mississippi), were used for intranasal (i.n.) and
intraperitoneal (i.p.) immunization of mice and respective antibody responses were compared. In ELISA,
using purified influenza A virus as antigen, the highest titer of antiviral antibodies was observed after a repeated
i.n. infection, in which the Dunedin strain was followed by the Mississippi strain and vice versa. Similarly, in
virus neutralization (VN) test, the highest titer of VN antibodies was found after a repeated i.n. infection. The
subunit vaccine INFLUVAC, when administered intramuscularly (i.m.), induced only a poor antibody response
as assayed by ELISA. Moreover, the INFLUVAC vaccination elicited a 100-fold lower titer of VN antibodies
than the i.n. infection and an approx a 10-fold lower titer than the i.p. immunization. A repeated INFLUVAC
vaccination did not lead to a significant increase of VN antibody titer. Also the antibody response to HA2gp –
a conserved part of influenza hemagglutinin (HA) that might contribute to the induction of specific antiviral
antibodies – was followed. Similarly to the VN antibody response, the highest HA2 antibody titer was induced
after a repeated i.n. infection, whereas the lowest HA2 antibody titer was observed after a single or repeated
INFLUVAC vaccination. Overall, the HA2 antibody titers remarkably well corresponded to the VN potential
of the examined sera.
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Keywords: antibodies; ELISA; influenza A virus; vaccine; INFLUVAC; virus neutralization |
Year: 2005, Volume: 49, Issue: |
Page From: 243, Page To: 250 |
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