Home FOR AUTHORS Endocrine Regulations 2006 Endocrine Regulations Vol.40, p.21-27, 2006

Journal info

Quarterly, 50 pp. per issue 
Founded: 1967
ISSN 1210-0668
E-ISSN 1336-0329

Published in English

Aims and Scope
Editorial Info
Submission Guidelines

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Endocrine Regulations Vol.40, p.21-27, 2006

Title: BINDING OF DOA KINASE TO SPECIFIC LOCI IN POLYTENE CHROMOSOMES OF DROSOPHILA MELANOGASTER
Author: MICHAELA KOVÁČIKOVÁ, IVAN RAŠKA, ANTON MATEÁŠIK, BRUCE A. CHASE, ROBERT FARKAŠ

Abstract: Objectives. Highly conserved LAMMER kinases belong to the family of dual-specific proteins phosphorylating enzymes with homologues in yeast, plants, Drosophila and mammals. SR proteins, several members of which are components of the spliceosomal complex and have been implicated in the control of alternative splicing, were identified among first targets of LAMMER kinases. The Drosophila locus Darkener of apricot (Doa) encodes best known representative of the LAMMER family of kinases, which is essential for embryonic and postembryonic development, neurogenesis, differentiation of photoreceptors, and sex determination. Methods. DOA kinase was detected on squash preparations of polythene chromosomes using anti-DOA antibodies in combination with indirect immunofluorescence. Results. Here we provide evidence for active chromosomal presence of DOA kinase in Drosophila salivary glands, and increased abundance of DOA at eleven specific loci of polythene chromosomes where it can be involved in on-site control of splicing process. Many genes that may be found at the loci with increased abundance fall into three major groups based on their known functions. First group contains genes that code for transcription factors, RNA-binding proteins or chromatin modifying enzymes. Second group of genes encode proteins which belong to proteasomal components, lipid enzymes, stress-sensitive ER proteins and caspases which are all early/proximal components of apoptotic process. Third group of genes comprises of tRNA coding genes or genes of tRNA synthases (ligases), all involved in protein synthesis. Some of the encoded protein products are confirmed or potential substrates of DOA kinase activity. Conclusions. Data indicate that genes encoding proteins involved in closely related pathways are concentrated at certain loci to achieve more efficient regulation of their expression as exemplified from distribution of DOA protein on Drosophila polythene chromosomes. For further details on this paper see also “www.elis.sk”.

Keywords: Dual – Specific protein kinases – Drosophila – LAMMER kinase – DOA – Subcellular localisation – Polytene chromosomes. – Chromatin on-site modification
Year: 2006, Volume: 40, Issue: 1 Page From: 21, Page To: 27



download file



© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.