Neoplasma Vol.53, p.434-439, 2006
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Title: Carboxymethyl chitin-glucan enriched diet exhibits protective
effects against oxidative DNA damage induced in freshly isolated
rat cells
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Author: M., LAZAROVA
; J., LABAJ
; G., KOGAN
; D., SLAMENOVA
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Abstract: The connection between dietary intake of carboxymethyl chitin-
glucan (CM-CG, approximately 200 mg/kg body
weight, during 21 days) and the response of freshly isolated rat
cells to genotoxic treatment with a combination of
photosensitizer Methylene Blue and visible light (MB+VL) was
evaluated in presented study. Blood lymphocytes, testicular
cells, and hepatocytes were isolated from rats fed by a standard
or CM-CG enriched diet and in ex vivo conditions challenged
with oxidative agent. Induced DNA damage was assessed using a
modified comet assay.
When added to the diet, CM-CG itself did not induce any negative
effect on the health condition of animals or on level of
DNA breaks in rat cells. Moreover, the cells isolated from CM-CG
fed animals were more resistant to oxidative stress induced
by visible light-excited Methylene Blue.
In conclusion, we have demonstrated that carboxymethyl chitin-
glucan represents a natural fungal polysaccharide that is
able to exert antimutagenic properties upon application in diet.
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Keywords: photosensitization, oxidative DNA lesions, carboxymethyl chitin-
glucan, diet, comet assay
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Year: 2006, Volume: 53, Issue: |
Page From: 434, Page To: 439 |
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