Neoplasma Vol.50, p.1-7, 2003
|
| Title: Different patterns of chromogranin A and Leu-7 (CD57) expression in gastrointestinal carcinoids: immunohistochemical and confocal laser scanning microscopy study
|
| Author: T., JIRASEK ; P., HOZAK ; V., MANDYS; |
|
Abstract: Thirty-seven carcinoids of the gastrointestinal tract were studied with
immunohistochemical staining for chromogranin A (CgA) and Leu-7 (CD57). The aim
of this study was to distinguish and describe the differences in patterns of
distribution of immunostaining of these two non-specific neuroendocrine markers
in neuroendocrine tumors of different degree of differentiation (typical, vs.
atypical carcinoids) at different gastrointestinal sites. Selected 5 tumors from
this group were studied in detail using confocal laser scanning microscopy
(CLSM) and double immunofluorescence staining to disclose the patterns of
distribution of CgA and CD57 positive granules within the individual tumor
cells. Prominent differences in the patterns of immunohistochemical staining for
both studied markers related to the degree of differentiation of the tumors were
observed in studied neoplasms. Regular (diffuse) strongly positive
immunoreaction for CgA predominated in typical carcinoids, whereas atypical
tumors were characterized by irregular patchy staining. Both typical and
atypical tumors displayed predominantly irregular patchy staining for CD57. The
results of CLSM study indicate that different modes of CgA and CD57 expression
and/or co-expression can occur in neuroendocrine tumors. Neoplastic cells that
contained either CgA positive neuroendocrine granules (NEG), or Leu-7 positive
NEG, were frequently observed in different areas of the tumor samples,
especially in atypical carcinoids. Varying number of cells revealed
co-localisation of both CgA and Leu-7 within the NEG. Similar co-localisation of
CgA and CD57 was found in non-neoplastic Kultschitski cells of the mucosa of
small intestine. In conclusion, our results suggest that the differences in CgA
and CD57 expression in human neuroendocrine tumors are related to the degree of
differentiation of the neoplasms and probably reflect the degree of maturation
(functional state) of neuroendocrine granules within the neoplastic cells.
|
|
| Keywords: Gastrointestinar carcinoid, chromogranin A, Leu-7 (CD57), immunohistochemistry, confocal laser scanning microscopy.
|
| Year: 2003, Volume: 50, Issue: |
Page From: 1, Page To: 7 |
|
|
|
|
|
 download file |
|
|
|
|
