General Physiology and Biophysics Vol.30, No.3, p.301

General Physiology and Biophysics Vol.30, No.3, p.301–309, 2011

Quarterly, 80 pp. per issue
Founded: 1982
ISSN 1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.30, No.3, p.301–309, 2011
Title: Biophoton emissions from cell cultures: biochemical evidence for the plasma membrane as the primary source
Author: Blake T. Dotta, Carly A. Buckner, Dianne Cameron, Robert M. Lafrenie, Michael A. Persinger

Abstract: Photon emissions were measured at ambient temperature (21°C) in complete darkness once per min from cultures of 106 cells during the 12 h following removal from 37°C. The energy of emission was about 10–20 J/s/cell. Of 8 different cell lines, B16-BL6 (mouse melanoma cells) demonstrated the most conspicuous emission profile. Acridine orange and ethidium bromide indicated the membranes were intact with no indication of (trypan blue) cell necrosis. Treatments with EGF and ionomycin produced rapid early (first 3 h) increases in energy emission while glutamine-free, sodium azide and wortmanin-treated cells showed a general diminishment 3 to 9 h later. The results suggested the most probable origin of the photon emission was the plasma cell membrane. Measures from cells synchronized at the M- and S-phase supported this inference.

Keywords: Biophoton emission — B16 (melanoma) cells — Membrane — EGF — Cell culture

Year: 2011, Volume: 30, Issue: 3	Page From: 301, Page To: 309
doi:10.4149/gpb_2011_03_301
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