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Acta Virologica Vol.55, No.3, p.235-241, 2011 |
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Title: Studies on interaction of cucurbit aphid-borne yellow virus proteins using yeast two-hybrid system and bimolecular fluorescence complementation | ||
Author: X. H. CHEN, H. Y. XIANG, Z. WANG, Y. J. ZHANG, C. G. HAN, D. W. LI, J. L. YU, Y. Q. CHENG | ||
Abstract: In this article, yeast two-hybrid system (YTHS) and bimolecular fluorescence complementation (BiFC) were used to analyze the interactions of cucurbit aphid-borne yellows virus (CABYV)-encoded proteins. P0, P1, P1-2, P3, P4, and P5 were tested by YTHS in all possible pairwise combinations, and only P3/P3 interaction was detected. Results obtained by BiFC further confirmed the self-interaction of P3, and the subcellular localization of reconstituted YFP fluorescence was observed mainly in nuclei of Nicotiana benthamiana leaf epidermal cells. Domains involved in P3/P3 self-interaction were analyzed by YTHS and BiFC using deletion mutants. The results showed that R domain (residues 1–61) in the N-terminus could self-interact, and it also interacted with the S domain (residues 62–199) in the C-terminus of P3. The present work would serve as a molecular basis for further characterization of CABYV proteins, and the regions involved in P3/P3 self-interaction could provide the clue for understanding the capsid assembly pathway of CABYV. |
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Keywords: Polerovirus; protein-protein interaction; subcellular localization; interaction domain | ||
Year: 2011, Volume: 55, Issue: 3 | Page From: 235, Page To: 241 | |
doi:10.4149/av_2011_03_235 |
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