Bratislava Medical Journal Vol.113, No.6, p.350–353, 2012
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Title: Protective role of vitamin C in diazepam-induced apoptosis in rat thymocytes |
Author: V. Pavlovic, D. Pavlovic, B. Kamenov, M. Sarac, Z. Peric, M. Velojic |
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Abstract: Abstract: Diazepam, a peripheral-type benzodiazepine receptor ligand, is widely used as a therapeutic agent. On the other hand, peripheral-type benzodiazepines have been shown to induce apoptosis in different immune cell types. In this study, we examined the possible protective role of vitamin C in diazepam-induced apoptosis and evaluated the cellular content of glutathione during this process. Rat thymocytes were incubated for 24 hours with diazepam and increasing concentrations of vitamin C or with diazepam alone. The exposure to diazepam resulted in an increase in apoptotic cell death and decrease in glutathione content in rat thymocytes. Vitamin C was effective in ameliorating the effect of diazepam in rat thymocytes by decreasing the proportion of apoptotic cells and increasing the cellular content of glutathione. These results suggest that vitamin C reduced the diazepam-induced apoptosis in rat thymocytes by restoring the cellular content of glutathione, which may be useful in preventing the diazepam-induced immunosupression (Tab. 1, Fig. 1, Ref. 31).
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Keywords: vitamin C, diazepam, thymocytes, apoptosis, glutathione. |
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Year: 2012, Volume: 113, Issue: 6 |
Page From: 350, Page To: 353 |
doi:10.4149/BLL_2012_079
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