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FOR AUTHORS General Physiology and Biophysics 2013 General Physiology and Biophysics Vol.32, No.3, p.371-380, 2013
FOR AUTHORS General Physiology and Biophysics 2013 General Physiology and Biophysics Vol.32, No.3, p.371-380, 2013
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General Physiology and Biophysics Vol.32, No.3, p.371-380, 2013 |
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| Title: The effect of probiotic Escherichia coli strain Nissle 1917 lipopolysaccharide on the 5-aminosalicylic acid transepithelial transport across Caco-2 cell monolayers | ||
| Author: Věra Štětinová, Libuše Smetanová, Dagmar Kholová, Jaroslav Květina, Zbyněk Svoboda, Zdeněk Zídek, Helena Tlaskalová-Hogenová | ||
| Abstract: The object of this study was to investigate the effect of probiotic Escherichia coli strain Nissle 1917 (EcN) (i) EcN lipopolysaccharide (EcN LPS) and (ii) bacteria-free supernatant of EcN suspension (EcN supernatant) on in vitro transepithelial transport of mesalazine (5-aminosalicylic acid, 5-ASA), the most commonly prescribed anti-inflammatory drug in inflammatory bowel disease (IBD). Effect of co-administered EcN LPS (100 µg/ml) or EcN supernatant (50 µg/ml) on the 5-ASA transport (300 µmol/l) was studied using the Caco-2 monolayer (a human colon carcinoma cell line) as a model of human intestinal absorption. Permeability characteristics for absorptive and secretory transport of parent drug and its intracellularly-formed metabolite were determined. The quantification of 5-ASA and its main metabolite N-acetyl-5-amino-salicylic acid (N-Ac-5-ASA) was performed by high performance liquid chromatography. Obtained results suggest that neither EcN LPS nor EcN supernatant had effect on the total 5-ASA transport (secretory flux greater than absorptive flux) and on the transport of intracellularly formed N-Ac-5-ASA (preferentially transported in the secretory direction). The percent cumulative transport of the total 5-ASA alone or in combination with EcN LPS or EcN supernatant did not exceed 1%. |
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| Keywords: Caco-2 cells — Probiotics — E. coli Nissle lipopolysaccharide — 5-Aminosalicylic acid — Drug transport | ||
| Year: 2013, Volume: 32, Issue: 3 | Page From: 371, Page To: 380 | |
| doi:10.4149/gpb_2013035 |
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