Home FOR AUTHORS General Physiology and Biophysics 2014 General Physiology and Biophysics Vol.33, No.2, p.227–234, 2014

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Quarterly, 80 pp. per issue
Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.33, No.2, p.227–234, 2014

Title: Crystallization, solubility and thermodynamics of the highly thermostable glucose isomerase from Streptomyces sp. strain
Author: Mohamed A. Borgi, Moez Rhimi, Adel Kadri
Abstract: The crystallization behaviour of the highly thermostable glucose isomerase from the Streptomyces sp. strain isolated from Tunisian soil was investigated using ammonium sulfate as a precipitating agent. We established phase diagrams at different temperatures and protein concentrations. It was found that the solubility increased with increasing temperature and decreased with increasing salt concentration. The temperature-dependent solubility was used to characterize the thermodynamic parameters of crystallization such as enthalpy, entropy and free energy.
Keywords: Glucose isomerase — Biocrystallization — Solubility — Phase diagrams — Thermodynamics
Year: 2014, Volume: 33, Issue: 2 Page From: 227, Page To: 234
doi:10.4149/gpb_2013077


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