Home HOME General Physiology and Biophysics 2014 General Physiology and Biophysics Vol.33, No.3, p.321–334, 2014

Journal info


Quarterly, 80 pp. per issue
Founded: 1982
ISSN  1338-4325 (online)

Published in English

Aims and Scope
Editorial Info
Abstracting and Indexing
Submission Guidelines

Select Journal







Webshop Cart

Your Cart is currently empty.

General Physiology and Biophysics Vol.33, No.3, p.321–334, 2014

Title: A chloride conductance exhibiting bicarbonate conductivity in renal inner medullary collecting duct cells
Author: Juan J. Bolívar, César O. Lara-Figueroa, Ramón H. Martínez-Mayorquin, Florencio Monroy-Romero, Gabina Arenas
Abstract: The anion conductance in primary cultures of rat inner medullary collecting duct cells was studied using perforated-patch whole-cell clamp technique. Depolarizations above 0 mV induced an outward anionic current with a time-dependent activation (Iovt) exhibiting a similar conductivity to Cl– and HCO3–. Iovt showed half-maximal activation around 32 mV with a slope factor of 23 mV, and showed a voltage-dependent activation time course that was well fitted by a sum of two exponential functions. Iovt was potentiated when external pH values or external Ca2+ concentration was increased and was blocked by external DIDS, DPC and furosemide. These characteristics of Iovt resemble that of the ClC-K1 channels-mediated currents; however, anion substitution studies showed that Iovt exhibits a Br– > Cl– > I– > NO3– conductivity sequence, different from that observed in the ClC-K1 channels-mediated conductance. We suggest that, in inner medullary collecting duct cells, ClC-K channels of an unidentified type give rise to this Cl– and HCO3– conductance. This is the first study of a channel-mediated HCO3– current in kidney tubular cells.
Keywords: Kidney — Anion channel — Bicarbonate transport — Cl– current — IMCD
Year: 2014, Volume: 33, Issue: 3 Page From: 321, Page To: 334
doi:10.4149/gpb_2014006


download file


© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.