Acta Virologica Vol.59, No.1, p.33-39, 2015
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Title: Production, characterization and immunogenicity of P particles derived from norovirus GII.4 genotype 2004 variant |
Author: W. Su, J. Gao, Y. Zang, H. Wu, L. Wang, H. Hu, X. Yu, W. Kong, C. Jiang |
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Abstract: Norovirus (NoV) is the main cause of nonbacterial infectious gastroenteritis. Due to the difficulty of culturing the virus, research on vaccine against NoV is focused on virus-like particles (VLPs). On the other hand, the P particles assembled from the P domains of NoV capsid protein become a promising vaccine candidate. GII.4 is the most prevalent genotype of NoV. While the immunogenicity of P particles derived from the GII.4 1996 variant has been investigated, the research on P particles of more recently prevalent variants is lacking. In this study, the P domain of the capsid protein of GII.4 genotype 2004 variant was expressed in Escherichia coli, purified and auto-assembled into P particles of 14–25 nm. Immunization with P particles induced specific serum antibodies with titers of 245,600 and 145,700 in mice and rabbits, respectively. The GII.4 NoV 2004 variant bound to type A, B and O secretor-positive saliva and immune sera blocked this binding, suggesting induction of neutralizing activity in such sera. Thus, this study demonstrated the immunogenicity of NoV P particles generated from E. coli and provided evidence supporting the development of this approach.
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Keywords: Norovirus; P particle; histo-blood group antigens |
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Year: 2015, Volume: 59, Issue: 1 |
Page From: 33, Page To: 39 |
doi:10.4149/av_2015_01_33
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