Home Bratislava Medical Journal 2015 Bratislava Medical Journal Vol.116, No.5, p.326-329, 2015

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

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Bratislava Medical Journal Vol.116, No.5, p.326-329, 2015

Title: The effect of nesfatin-1 on heart L-type Ca2+ channel α1c subunit in rats subjected to chronic restraint stress
Author: C. Ayada, G. Turgut, S. Turgut

Abstract:

Background: Elevated L-type Ca2+ channel expression level increases Ca2+ influx. This can cause hypertrophy and pathological remodeling of the heart especially under stress conditions. Nesfatin-1 can activate hypothalamic L, P and Q type Ca2+ channels and increase insulin secretion in pancreatic islet beta cells via activation of L-type Ca2+ channels. On the other hand, the effect of nesfatin-1 on cardiac L-type Ca2+ channels has not been studied yet.
Objectives: We aimed to identify the effect of peripheral chronic nesfatin-1 application on cardiac L-type Ca2+ channel α1c subunit expression level in normal rats and those subjected to chronic restraint stress.


Methods: Three-month aged Wistar albino rats were randomly divided into 4 groups (n = 7) as Control, Stress, Control+Nesfatin-1, and Nesfatin-1+Stress.

Rats in groups subjected to restraint stress were placed in a specially built size-manipulable cabin for 2 h/day (between 10:00 and 12:00 a.m.) for 10 consecutive days without allowing water and food intake. Nesfatin-1 segment (0.25 nmol/g bw intraperitoneally) was applied during the 10 consecutive days. Western blot analyses were performed to determine the expression level of L-type Ca2+ channel α1c subunit protein in rat cardiac extracts.

Results: Cardiac L-type Ca2+ channel α1c subunit protein expression levels were increased significantly after chronic peripheral nesfatin-1 application in rats subjected to restraint stress (p = 0.032).


Conclusion: We can conclude that nesfatin-1 can cause cardiac failures during clinical treatments by elevating cardiac L-type Ca2+ channel α1c subunit protein expression level (Fig. 2, Ref. 26).



Keywords: nesfatin-1, chronic restraint stress, cardiac failure, L-type Ca2+ channel α1c subunit, Western blot.
Year: 2015, Volume: 116, Issue: 5 Page From: 326, Page To: 329
doi:10.4149/BLL_2015_061


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