Home Bratislava Medical Journal 2016 Bratislava Medical Journal Vol.117, No.5, p.268-271, 2016

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Published Monthly, in English
Founded: 1919
ISSN 0006-9248
(E)ISSN 1336-0345

Impact factor 1.2

 

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Bratislava Medical Journal Vol.117, No.5, p.268-271, 2016

Title: Freund´s complete adjuvant effect on BALB/c mice: an insight into inflammation and oxidative stress after immunity challenge
Author: M. Pohanka, I. Vobornikova, J. Fusek

Abstract: Freund´s complete adjuvant (FCA) is a mean used for improving immunization efficacy in experiments and veterinary medicine. Despite high efficacy, it is not used in human vaccination due to number of adverse effects. Arthritis can be an example of a typical adverse consequence. In this work, we decided to investigate the link between oxidative stress and inflammation in animals exposed to FCA. BALB/c mice received either saline or FCA in doses 10–50 µl and were euthanized from one to three days after infection. Interleukins (IL) IL-2, IL-4 and tumor necrosis factor α (TNF-α) were determined in plasma. Ferric reducing antioxidant power (FRAP), malondialdehyde by thiobarbituric acid reactive substances assay (TBARS), reduced glutathione (GSH) and caspase-3 were measured in livers, muscles and other organs. We determined significant effect of FCA on TBARS and GSH in the livers. Beside this, TBARS were altered in muscles as well. Inflammation was confirmed by increased level of TNF-α. Taking the results into account, we infer that the harmful effect of FCA is mediated not only by inflammation but also by oxidative. This should be considered when pathological consequences of FCA are scored (Fig. 4, Ref. 21).

Keywords: adjuvant, oxidative stress, inflammation; glutathione, immunity, vaccination, arthritis
Published online: 13-May-2016
Year: 2016, Volume: 117, Issue: 5 Page From: 268, Page To: 271
doi:10.4149/BLL_2016_052


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