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Bratislava Medical Journal 2016 Bratislava Medical Journal Vol.117, No.7, p.376-380, 2016
Bratislava Medical Journal 2016 Bratislava Medical Journal Vol.117, No.7, p.376-380, 2016
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Bratislava Medical Journal Vol.117, No.7, p.376-380, 2016 |
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| Title: The role of miR-146a on NF-κB expression level in human umbilical vein endothelial cells under hyperglycemic condition | ||
| Author: K. Kamali, E. Salmani Korjan, E. Eftekhar, K. Malekzadeh, F. Ghadiri Soufi | ||
| Abstract: Emerging studies have been shown that the expression of micrRNA-146a (miR-146a, as a regulator of nuclear factor κB (NF-κB)), is changed in diabetic patients and animals. This study was designed to evaluate the possible role of miR-146a in the pathogenesis of diabetes-related microvascular complications. Concurrent with the creation of cellular hyperglycemia (25 mmol/L for 24 h), human umbilical vein endothelial cells (HUVECs) were transfected with 20 nmol/L of hsa-miR-146a antagomir or scramble using HiPerFect reagent (Qiagen). D-mannitol was used as osmotic control. Hyperglycemia increased the NF-κB gene expression and protein activity (as an inflammation index) in cultured HUVECs. Moreover, the gene expression level of miR-146a, and its target proteins, tumor necrosis factor receptor-associated factor 6 (TRAF6) and interleukin-1 receptor-associated kinase 1 (IRAK1) were increased under hyperglycemic condition. The knockdown of miR-146a by transfection of miR-146a antagomir notably increased the NF-κB activity and decreased the NF-κB mRNA in hyperglycemic HUVECs. Furthermore, miR-146a antagomir significantly increased IRAK1 and TRAF6 mRNA levels under hyperglycemic condition. These results demonstrate that the expression of miR-146a is upregulated in HUVECs during early phase of hyperglycemic condition possibly to regulate the NF-κB activity through inhibition of IRAK1 and TRAF6 (Fig. 4, Ref. 32). |
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| Keywords: hyperglycemia, HUVECs, miRNA-146a, NF-κB, IRAK1, TRAF6 | ||
| Published online: 12-Jul-2016 | ||
| Year: 2016, Volume: 117, Issue: 7 | Page From: 376, Page To: 380 | |
| doi:10.4149/BLL_2016_074 |
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