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Bratislava Medical Journal 2017 Bratislava Medical Journal Vol.118, No.8, p.443-448, 2017
Bratislava Medical Journal 2017 Bratislava Medical Journal Vol.118, No.8, p.443-448, 2017
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Bratislava Medical Journal Vol.118, No.8, p.443-448, 2017 |
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| Title: The effect of leptin and resveratrol on JAK/STAT pathways and Sirt-1 gene expression in the renal tissue of ischemia/reperfusion induced rats | ||
| Author: S. Erkasap, N. Erkasap, B. Bradford, L. Mamedova, O. Uysal, M. Ozkurt, R. Ozyurt, O. Kutlay, B. Bayram | ||
| Abstract: OBJECTIVE: Our study aimed to investigate the possible modifying effects of leptin and combined use of resveratrol on rat renal I/R injury and their relationship on signal pathways and apoptosis-related mechanisms. BACKGROUND: Renal ischemia-reperfusion (I/R) injury is an important cause of acute renal failure. METHODS: Male Sprague Dawley rats were divided into 5 groups: Control, I/R, I/R+leptin, I/R+resveratrol and I/R+leptin+resveratrol. Leptin (10 μg/kg BW) was administered (i.p.) 30 min prior to I/R. Resveratrol was administered by gavage at 20 mg/kg BW per d for 12 d prior to I/R. The left renal artery was exposed to 1 h of ischemia and 1 h of reperfusion. RESULTS: Resveratrol treatment alone increased TNF-α, TNF-α R1, NF-κB, SIRT-1, STAT1 and STAT3 mRNA levels and decreased caspase 3 protein levels. Leptin treatment alone significantly decreased the caspase 3 protein levels. The combined use of resveratrol and leptin significantly increased STAT3, and caspase 3 mRNA levels, and decreased the caspase 3 protein levels. Apoptosis was significantly decreased especially in the leptin and leptin+resveratrol groups. CONCLUSION: The present study suggest that a combined use of resveratrol and leptin has preventive and regulatory effects on renal I/R injury; the mechanism involves decreasing apoptosis, likely by altering the JAK/STAT pathway and SIRT1 expression (Fig. 8, Ref. 24). |
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| Keywords: leptin, resveratrol, TNF-alpha, caspase 3, apoptosis | ||
| Published online: 31-Aug-2017 | ||
| Year: 2017, Volume: 118, Issue: 8 | Page From: 443, Page To: 448 | |
| doi:10.4149/BLL_2017_086 |
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