Home Acta Virologica 2018 Acta Virologica Vol.62, No.3, p.235-245, 2018

Journal info


Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

Aims and Scope
Abstracted and Indexed

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Acta Virologica Vol.62, No.3, p.235-245, 2018

Title: α2,3- and α2,6-linked sialic acids are important for cell binding and replication of Newcastle disease virus in chicken primary neuronal cells
Author: Y. GUO, J. ZHAO, X. CHANG, W. YAO, H. WANG, W. WANG, X. WANG, S. ZHANG, Z. YANG, S. XIAO

Abstract: Velogenic Newcastle disease virus (NDV) causes encephalitis and severe neurological disorders in avian species. Sialic acid (SA) has been considered as a receptor for NDV infection and determining tissue tropism. The neurotropic mechanism of NDV in birds is completely unknown. Here we have investigated the role of viral receptor SA in neurotropism of NDV in chickens. We determined that α2,3- and α2,6-linked SA receptors were implicated in NDV encephalitis and viral binding to primary neuronal cells using immunohistofluorescence and virus-cell binding assay. Both SA receptors were found to co-localize with velogenic strain F48E9 in neuropathological lesions of chicken brains after infection through intraocular-nasal routes. The replication of velogenic F48E9 in primary neuronal cells was more efficient than that of lentogenic strain LaSota. The virus-neuronal cell binding capabilities of both the velogenic and the lentogenic strains have no difference. Furthermore, the cell-binding capability and the replication of both strains were significantly decreased by pretreatment with sialidases in neuronal cells. These results demonstrated that α2,3- and α2,6-linked SA receptors were important for the initiation of NDV infection in the chicken nervous system. This study should provide preliminary evidence for a better understanding of the neurotropism of NDV in chickens.

Keywords: Newcastle disease virus; sialic acid; chicken; primary neuronal cells
Published online: 30-Aug-2018
Year: 2018, Volume: 62, Issue: 3 Page From: 235, Page To: 245
doi:10.4149/av_2018_217


download file



© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.