Home Endocrine Regulations 2008 Endocrine Regulations Vol.42, p.137-146, 2008

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Quarterly, 50 pp. per issue 
Founded: 1967
ISSN 1210-0668
E-ISSN 1336-0329

Published in English

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Endocrine Regulations Vol.42, p.137-146, 2008

Title: FOS EXPRESSION IN HYPOCRETINERGIC NEURONS IN C57B1/6 MALE AND FEMALE MICE AFTER LONG-TERM CONSUMPTION OF HIGH FAT DIET
Author: Z. PIRNIK, J. BUNDZIKOVA, J. D. MIKKELSEN, B. ZELEZNA, L. MALETINSKA, A. KISS

Abstract: Objective. It is generally known that hypocretin (Hcrt) neurons in lateral hypothalamus (LH) are involved in feeding behaviour. The aim of this study was to reveal the activity response of Hcrt neurons, as measured by Fos protein incidence, to prolonged high fat (HF) diet in the LH of both genders of C57B1/6 mice. Methods. Standard (St) and high fat (HF) diets were available to mice for 16 weeks and thereafter the animals were perfused transcardially with fixative. Then the brains were removed, soaked with 15 % sucrose in 0.1 M phosphate buffer (PB), and cryo-sectioned throughout the hypothalamus into 35 μm thick coronal sections. Fos/Hcrt co-localizations were processed by employing avidin-biotin-peroxidase (ABC) complex and diaminobenzidine chromogen for Hcrt labeling. Fos immunoproduct was intensified by nickel chloride as a black color inducer. Evaluation of the incidence of Fos/Hcrt co-labeled perikarya was performed using a computerized Leica light microscopy. Results. The effect of of mice gender, applied diet, and gender plus applied diet on the activation of Hcrt neurons was found. Turkey´s test revealed significant (p

Keywords: Fos - Hypocretin neurons - Lateral hypothalamus - High fat diet - Male and female C57B1/6 mice
Year: 2008, Volume: 42, Issue: 4 Page From: 137, Page To: 146



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